Minimate TFF capsule - Sample handling - 제이에스텍(JS-TECH)

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관리자 2021-10-26 14:59:28

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The easy to use Minimate TFF capsule is a valuable tool for any laboratory requiring tangential flow filtration of small process volumes. Sample batch sizes of up to one liter can be reduced to as low as 5 mL. Sample concentration and diafiltration (desalting) can take place sequentially using the same device and system, saving time and avoiding product loss associated with transfer steps.
The Minimate TFF Capsules contain a robust, polyethersulfone, ultrafiltration (UF) membrane. The Omega UF membrane offers low non-specific adsorption characteristics resulting in high product recovery. The Minimate TFF capsules are available with a wide range of molecular weight cut-offs. They can be used in a variety of biological applications in biotechnology, molecular biology, protein chemistry, microbiology and immunology.

Features

Ready to use – Minimate TFF capsule
includes all fittings and tubing to
simplify installation into filtration setups.
This versatile, self-contained device works with most laboratory pumps Cost-effective design – The plastic construction of the Minimate TFF capsule and chemical compatibility of the Omega membrane facilitate cleaning and reuse
Scalable – The Minimate TFF capsules have the same path length and similar materials of construction as larger Centramate and Centrasette cassettes used in pilot and production scale. Several Minimate TFF capsules may be connected in parallel for increased membrane area Predictable performance saves time when scaling up a process.
High Product Recovery – Optimized flow path design coupled with low protein binding Omega membrane minimize non-specific binding
Efficient – Concentration and desalting processes can be performed on the same system with minimal user intervention. Achieve high concentration factors in a single processing step due to the low hold-up volume of the device
Disposable – This economically priced device can be dedicated to a single batch or process eliminating the time required for cleaning
Confidence – Each pharmaceutical grade Minimate capsule is 100% integrity tested during manufacture to ensure reliable performance. For critical applications the user can re-test the integrity after initial use. A Certificate of Quality is included with each capsule

Applications

Concentrate and desalt proteins, peptides, or nucleic acids (DNA, RNA, oligonucleotides)

Recover antibodies or recombinant proteins from clarified cell culture media

Process metal sensitive enzymes and other biomolecules

Separate (fractionate) large from small biomolecules

Concentrate viruses or gene therapy vectors

Prepare samples prior to column chromatography

Concentrate samples after gel filtration

Depyrogenate water, buffers, and media solutions

Technical Data

Effective Filtration Area     50 cm² (0.05 ft²)
Recommended Crossflow     60 – 70 mL/min
Operating Temperature Range     5 – 50 °C (41 – 122 °F)
Maximum Operating Pressure     4 bar (400 kPa, 60 psi)
pH Range      1 – 14
Product Hold-up Volume     approximately 1.6 mL
Forward Flow Air integrity Value     ≤ 7/mL/min @ 0.75 bar (10 psi)
Nominal Dimensions     (L x W x H nominal):  200 mm x 380 mm x 180mm (8 in x 1.5 in x 0.7 in)

Materials of Construction

Filter media     Omega membrane (polyethersulfone)
Housing     Polypropylene, Glass reinforced
Screens, housing sealing ring, fittings      Polypropylene
Membrane plate/filtrate channels     Polyethylene
Internal gasket     Silicone

Minimate TFF Capsule Filters

This is a list of typical part numbers for the product described in this catalogue.
Click Part Number Configuration to access a PDF of the part numbering structure.
For part numbers and configurations that are that are not listed, please contact your Pall representative.

Part Number Configuration

Part Number	Description	Pkg
OAD65C12	Minimate TFF Capsule with 650 Da Omega membrane	1/pkg
OA001C12	Minimate TFF Capsule with 1 kDa Omega membrane	1/pkg
OA003C12	Minimate TFF Capsule with 3 kDa Omega membrane	1/pkg
OA005C12	Minimate TFF Capsule with 5 kDa Omega membrane	1/pkg
OA010C12	Minimate TFF Capsule with 10 kDa Omega membrane	1/pkg
OA030C12	Minimate TFF Capsule with 30 kDa Omega membrane	1/pkg
OA050C12	Minimate TFF Capsule with 50 kDa Omega membrane	1/pkg
OA070C12	Minimate TFF Capsule with 70 kDa Omega membrane	1/pkg
OA100C12	Minimate TFF Capsule with 100 kDa Omega membrane	1/pkg
OA300C12	Minimate TFF Capsule with 300 kDa Omega membrane	1/pkg
OA500C12	Minimate TFF Capsule with 500 kDa Omega membrane	1/pkg
OA990C12	Minimate TFF Capsule with 1000 kDa Omega membrane	1/pkg

The Minimate TFF Capsule comes with threaded luer plugs in each of the four ports.
A fitting package with the following components is included.
Accessories

Part Number and Description
88216 Minimate Fittings Kit
Contains:
2 Male luer to 3.2 mm (1⁄8 in.) hose barb
2 Female luer to 3.2 mm (1⁄8 in.) hose barb
120 cm (3.9 ft) Tubing 3.2 mm (1⁄8 in.) i.d.
1 Retentate screw clamp
4 Tubing clamps
1 Adhesive strips (loop and hook)